High Resolution Melt Parameter Considerations for Optimal Data Resolution
نویسندگان
چکیده
<0.02°C temperature increments on a system with <0.02°C uniformity variability (Tesoriero 2008). However, successful discrimination of sequence variants, including the most difficult-to-detect class IV SNPs, is not solely dependent on the temperature increments from which melt data are collected. Several factors influence DNA melting behavior, including reaction mix composition, amplicon size, GC content, and DNA concentration.
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